Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina

Isoelectric focusing.

Capillary Isoelectric Focusing

Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press...

Isoelectric focusing in a drop.

A novel approach to molecular separations is investigated using a technique termed droplet-based isoelectric focusing. Drops are manipulated discretely on a superhydrophobic surface, subjected to low voltages for isoelectric focusing, and split-resulting in a preparative separation. A universal indicator dye demonstrates the generation of stable, reversible pH gradients (3-10) in ampholyte buff...

Phosphorylation of photolyzed rhodopsin is calcium-insensitive in retina permeabilized by a-toxin (rhodopsin kinaseyphototransductionyphotoreceptor cellsycGMP)

Light triggers the phototransduction cascade by activating the visual pigment rhodopsin (Rho 3 Rho*). Phosphorylation of Rho* by rhodopsin kinase (RK) is necessary for the fast recovery of sensitivity after intense illumination. Ca21 ions, acting through Ca21-binding proteins, have been implicated in the desensitization of phototransduction. One such protein, recoverin, has been proposed to reg...

Substitution of porphyropsin for rhodopsin in mouse retina.

Weanling, male albino mice were placed on a vitamin A-free diet for three months to deplete their vitamin A stores. The vitamin A-deficient mice were injected intraperitoneally with all-trans 3-dehydroretinol. 3-dehydroretinol was rapidly incorporated into the liver as a fatty acid ester. The chromophore of visual pigment increased gradually and reached a normal level 13 days after the injectio...